Figure 4.
Transient Transfection In Vitro
(A) IFN-γ secreted in culture supernatant by 3 × 105 A549 alveolar epithelial cells transfected with about 1 ng (filled symbols) or 1.5 ng (open symbols) of plasmid DNA. Means ± SD of three replicates are plotted. (B) A chimeric GFP-IFN-γ protein was not secreted, but it could be detected by flow cytometry. Means ± SD of three replicates are plotted. (C) The green fluorescence attributable to the chimeric protein co-localized with LysoTracker red at 48 h post-transfection to generate yellow fluorescence. (D) Detail of (C).