Fig. 5.

Quality control practices to manage and maintain the HELP biobank, and experimental applications. A. A visual representation of the dissected pieces of explanted heart tissues on a metal plate full of ice. B. Representative pictures showing optimized biopreservation methods leading to appropriately prepared TFM sample (1), paraffin-embedded blocks were systematically color-coded based on the structures (2), buffers for isolating cardiac cells or fixing tissues for histological examination (3), and embedded myocardial samples for ultrastructural study using an electron microscope (4). C. Experimental applications on the heart samples such as immunofluorescent light microscopy and confocal microscopy. D. A capture of the cryogenic long-term storage facility for appropriate depositing of different tissue samples. E. Systems biology analysis using advanced multi-omics techniques. Tissue sampling and processing (1), transcriptome from cells and nuclei delineating whole cardiac cell profiles by UMAP (2), spatial visualization of cell types probed by RNAscope (3), distribution of specific cell population after subclustering analysis (4). Modified from Litvinukova et al. [37] and reproduced with permission from Nature.