Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (D. melanogaster) | Hand4.2-Gal4 | NA | PMID:16467358 | NA |
| Genetic reagent (D. melanogaster) | UAS-trolRNAi | Vienna Drosophila Resource Center (VDRC) | FBst0454629 | v22642 |
| Genetic reagent (D. melanogaster) | UAS-CG1943RNAi | Vienna Drosophila Resource Center (VDRC) | FBst0453803 | v20758 |
| Genetic reagent (D. melanogaster) | UAS-apolppRNAi | Vienna Drosophila Resource Center (VDRC) | FBst0470481 | v6878 |
| Genetic reagent (D. melanogaster) | UAS-Hs6stRNAi | Vienna Drosophila Resource Center (VDRC) | FBst0464695 | v42658 |
| Genetic reagent (D. melanogaster) | UAS-mglRNAi | Vienna Drosophila Resource Center (VDRC) | FBst0461660 | v36389 |
| Genetic reagent (D. melanogaster) | UAS-SdhdRNAi | Vienna Drosophila Resource Center (VDRC) | FBst0456581 | v26776 |
| Genetic reagent (D. melanogaster) | UAS-Nhe2RNAi | Vienna Drosophila Resource Center (VDRC) | FBst0477879 | v106053 |
| Genetic reagent (D. melanogaster) | UAS-JupiterRNAi | Vienna Drosophila Resource Center (VDRC) | FBst0455704 | v25044 |
| Genetic reagent (D. melanogaster) | UAS-Eip74EFRNAi | Vienna Drosophila Resource Center (VDRC) | FBst0477129 | v105301 |
| Genetic reagent (D. melanogaster) | UAS-Sik2RNAi | Vienna Drosophila Resource Center (VDRC) | FBst0456442 | v26496 |
| Genetic reagent (D. melanogaster) | mglMI14318 | Bloomington Drosophila Stock Center (BDSC) | FBal0302551 | BL-59689 |
| Genetic reagent (D. melanogaster) | tin346 | NA | FBal0035787 | NA |
| Strain, strain background (D. rerio) | Oregon AB wild-type | A commonly used wild-type strain | ||
| Strain, strain background (D. rerio) | Tg(myl7:EGFP)twu277 | Tsai Lab, National Taiwan University | PMID:12950077 | A transgenic line of zebrafish labeled with heart-specific EGFP fluorescence. |
| Strain, strain background (D. rerio) | Tg(myl7:H2A-mCherry)sd12 | Yelon Lab, University of California, San Diego | PMID:24075907 | A transgenic line of zebrafish specifically expressing mCherry in cardiomyocyte nuclei |
| Antibody | mouse monoclonal anti-ACTN1 | Sigma | A7811 | 1:800 |
| Antibody | donkey polyclonal anti-mouse Alexa fluor 568 | Invitrogen | A10037 | 1:1000 |
| Antibody | chicken polyclonal anti-GFP | Aves Labs | GFP-1020 | 1:300 |
| Antibody | rabbit polyclonal abit-mCherry | Rockland | 600–401 P16S | 1:200 |
| Antibody | donkey polyclonal anti-chicken AlexaFluor 488 | Jackson ImmunoResearch | 703-545-155 | 1:200 |
| Antibody | donkey polyclonal anti-rabbit AlexaFluor 568 | Invitrogen | A10042 | 1:200 |
| Other | DAPI (iPSC) 500 mg/mL | Sigma | D9542 | 1:1000 |
| Other | DAPI (Zebrafish) 500 mg/mL | Invitrogen | D1306 | 1:200 |
| Sequence-based reagent | LRP2 siRNA | Entrez Gene ID: 4036 | Dharmacon | On-Target plus, SmartPool |
| Sequence-based reagent | APOB siRNA | Entrez Gene ID: 338 | Dharmacon | On-Target plus, SmartPool |
| Sequence-based reagent | PTCH1 siRNA | Entrez Gene ID: 5727 | Dharmacon | On-Target plus, SmartPool |
| Sequence-based reagent | TP53 siRNA | Entrez Gene ID: 7157 | Dharmacon | On-Target plus, SmartPool |
| Sequence-based reagent | CDKN1A siRNA | Entrez Gene ID: 1026 | Dharmacon | On-Target plus, SmartPool |
| Sequence-based reagent | ELF4 siRNA | Entrez Gene ID: 2000 | Dharmacon | On-Target plus, SmartPool |
| Sequence-based reagent | JPT1 siRNA | Entrez Gene ID: 51155 | Dharmacon | On-Target plus, SmartPool |
| Sequence-based reagent | HS6ST2 siRNA | Entrez Gene ID: 90161 | Dharmacon | On-Target plus, SmartPool |
| Sequence-based reagent | HSPG2 siRNA | Entrez Gene ID: 3339 | Dharmacon | On-Target plus, SmartPool |
| Sequence-based reagent | PRTG siRNA | Entrez Gene ID: 283659 | Dharmacon | On-Target plus, SmartPool |
| Sequence-based reagent | SDHD siRNA | Entrez Gene ID: 6392 | Dharmacon | On-Target plus, SmartPool |
| Sequence-based reagent | SIK1 siRNA | Entrez Gene ID: 150094 | Dharmacon | On-Target plus, SmartPool |
| Sequence-based reagent | SLC9A1 siRNA | Entrez Gene ID: 6548 | Dharmacon | On-Target plus, SmartPool |
| Sequence-based reagent | CDH | Hs00170423_m1 | IDT Integrated DNA technologies, Coralville, IA | characterization of the pluripotent state |
| Sequence-based reagent | DNMT3 | Hs01003405_m1 | IDT Integrated DNA technologies, Coralville, IA | characterization of the pluripotent state |
| Sequence-based reagent | DPPA2 | Hs00414521_g1 | IDT Integrated DNA technologies, Coralville, IA | characterization of the pluripotent state |
| Sequence-based reagent | DPPA5 | Hs00988349_g1 | IDT Integrated DNA technologies, Coralville, IA | characterization of the pluripotent state |
| Sequence-based reagent | ERAS | Hs.PT.45.4849266.g | IDT Integrated DNA technologies, Coralville, IA | characterization of the pluripotent state |
| Sequence-based reagent | GDF3 | Hs00220998_m1 | IDT Integrated DNA technologies, Coralville, IA | characterization of the pluripotent state |
| Sequence-based reagent | OCT-4 | Hs.PT.45.14904310.g | IDT Integrated DNA technologies, Coralville, IA | characterization of the pluripotent state |
| Sequence-based reagent | REXO1 | Hs.PT.45.923095.g | IDT Integrated DNA technologies, Coralville, IA | characterization of the pluripotent state |
| Sequence-based reagent | SALL4 | Hs00360675_m1 | IDT Integrated DNA technologies, Coralville, IA | characterization of the pluripotent state |
| Sequence-based reagent | TDG1 | Hs02339499_g1 | IDT Integrated DNA technologies, Coralville, IA | characterization of the pluripotent state |
| Sequence-based reagent | TERT | Hs99999022_m1 | IDT Integrated DNA technologies, Coralville, IA | characterization of the pluripotent state |
| Sequence-based reagent | APOB | Hs.PT.56a.1973344 | IDT Integrated DNA technologies, Coralville, IA | characterization of the pluripotent state |
| Sequence-based reagent | DHCR24 | Hs.PT.56a.4561516 | IDT Integrated DNA technologies, Coralville, IA | expression during guided cardiac differentiation |
| Sequence-based reagent | ELF4 | Hs.PT.56a.25941471 | IDT Integrated DNA technologies, Coralville, IA | expression during guided cardiac differentiation |
| Sequence-based reagent | HN1 | Hs.PT.58.40922463.g | IDT Integrated DNA technologies, Coralville, IA | expression during guided cardiac differentiation |
| Sequence-based reagent | HSPG2 | Hs.PT.56a.18698732 | IDT Integrated DNA technologies, Coralville, IA | expression during guided cardiac differentiation |
| Sequence-based reagent | HS6ST2 | Hs.PT.56a.1354985 | IDT Integrated DNA technologies, Coralville, IA | expression during guided cardiac differentiation |
| Sequence-based reagent | LRP2 | Hs.PT.56a.1584067 | IDT Integrated DNA technologies, Coralville, IA | expression during guided cardiac differentiation |
| Sequence-based reagent | MYLK | Hs.PT.56a.39795491 | IDT Integrated DNA technologies, Coralville, IA | expression during guided cardiac differentiation |
| Sequence-based reagent | PCDH11X | Hs.PT.56a.26531358 | IDT Integrated DNA technologies, Coralville, IA | expression during guided cardiac differentiation |
| Sequence-based reagent | PRTG | custom design | IDT Integrated DNA technologies, Coralville, IA | expression during guided cardiac differentiation |
| Sequence-based reagent | SIK1 | Hs.PT.58.2995158 | IDT Integrated DNA technologies, Coralville, IA | expression during guided cardiac differentiation |
| Sequence-based reagent | SLC9A1 | Hs.PT.58.15072523 | IDT Integrated DNA technologies, Coralville, IA | expression during guided cardiac differentiation |
| Sequence-based reagent | SDHD | Hs.PT.58.40267655.g | IDT Integrated DNA technologies, Coralville, IA | expression during guided cardiac differentiation |
| Sequence-based reagent | GAPDH | Hs.PT.45.8326 | IDT Integrated DNA technologies, Coralville, IA | expression during guided cardiac differentiation |
| Commercial assay or kit | EdU | Click-it Plus EdU Imaging Kit | Life Technologies | |
| Chemical compound, drug | BIO (GSK-3 Inhibitor) | Sigma | B1686 | |
| Software, algorithm | Prism v7 and v8 | GraphPad Software |