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. 2020 Oct 16;9:e55212. doi: 10.7554/eLife.55212

Figure 10. Unloading of the hindlimbs in growing rats by either sciatic nerve resection or by tape restriction.

Sciatic nerve resection (A–E) or leg restriction with a kinesiology tape (F–J) was done at 10 days of age and both hindlimbs (manipulated and intact) were analyzed at day 23. For (A–E) sham-operated littermates were used as a control (only right hindlimb was utilized), whereas for (F–J) unmanipulated littermates were used as a control (only right hindlimb was utilized). (A–C, F–H) Representative Safranin O and Fast Green stained images of the knee joint. Black dash lines outline the SOC and solid lines outline the epiphysis area in A and F. Quantification of SOC size (normalized to total epiphysis size) (D, I) and bone mass (within the primary spongiosa region) (E, J). Bars are means ±95% confidential interval and every dot represent an individual animal, two-tailed Student’s t-test. ns, not significant.

Figure 10.

Figure 10—figure supplement 1. Footprint analysis revealed a clear difference between the right hindlimb of the sciatic nerve-resected and the sham-operated rats.

Figure 10—figure supplement 1.

Figure 10—figure supplement 2. Cartilage calcification but absence of the SOC formation in the newt growing on land.

Figure 10—figure supplement 2.

(A) Alcian blue and von Kossa staining of the humerus of the newt (Notophthalmus viridescens) revealed calcified chondrocytes at the post metamorphosis transient land stage. (B–C) TUNEL (B) and DNA replication licensing factor MCM2 (C) staining of the same salamander species revealed no notiecable differences in apoptotic and proliferating chondrocytes at the three developmental stages.