(A–P) Alcian blue and alizarin red staining of the femur (A–D), humerus (E–H), metatarsal (I–L), and metacarpal (M–P) of Jaculus jaculus (three-toed jerboa) at various postnatal (P) time points. The dashed white lines outline the SOC. The gait pattern was derived from our weekly observations (see Figure 3—videos 1 and 2). (Q–R) Ossification of the autopod (Q) and stylopod and zeugopod (R) of Jaculus jaculus (three-toed jerboa) at various postnatal stages. Data are means ± SD, representing inter-individual variation, one-way ANOVA. In (Q–R), n = 2 jerboa analyzed. ns, not significant.