FIGURE 6.

Determination of target genes related to the differentially expressed miRNAs (miR‐3679‐5p, miR‐6515‐5p, and miR‐6747‐5p) in Exs‐Ad‐P2X7. A, Osteogenic differentiation‐related signaling molecule expression of PDLSCs (qRT‐PCR assay) in inflammatory culture conditions in the presence of Exs‐control, Exs‐PDLSCs, Exs‐Ad‐P2X7, or Exs‐Ad‐control following a 7‐day incubation in an inflammatory osteogenic microenvironment. B, GREM‐1 gene expression (qRT‐PCR assay) and, C, GREM‐1 protein expression (Western blot analysis) of PDLSCs under inflammatory culture conditions in the presence of Exs‐control, Exs‐PDLSCs, Exs‐Ad‐P2X7, or Exs‐Ad‐control following a 7‐day incubation in an inflammatory osteogenic microenvironment. D, GREM‐1 gene expression (qRT‐PCR assay) and GREM‐1 protein expression (Western blot analysis) of PDLSCs following transfection with miR‐mimic NC, miR‐3679‐mimic, miR‐6515‐mimic, or miR‐6747‐mimic and a 7‐day incubation in an inflammatory osteogenic microenvironment. Relative luciferase activity of the GREM‐1 protein in, F, miR‐3679‐mimic‐transfected, G, miR‐6515‐mimic‐transfected, or, H, miR‐6747‐mimic‐transfected cells. Data are presented as the mean ± SD for n = 3; *P < .05, **P < .01, and ***P < .001 indicate significant differences between the indicated groups. PDLSC, periodontal ligament stem cell