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. Author manuscript; available in PMC: 2021 Feb 5.
Published in final edited form as: Nature. 2020 Aug 5;586(7830):567–571. doi: 10.1038/s41586-020-2622-0

Figure 1. MERS-CoV S-2P mRNA protects mice from lethal challenge.

Figure 1.

288/330+/+ mice were immunized at weeks 0 and 3 with 0.01 (green), 0.1 (blue), or 1 μg (red) of MERS-CoV S-2P mRNA. Control mice were administered PBS (gray). Two weeks post-boost, sera were collected from 3 mice per group and assessed for neutralizing antibodies against MERS m35c4 pseudovirus (a). Four weeks post-boost, 12 mice per group were challenged with a lethal dose of mouse-adapted MERS-CoV (m35c4). Following challenge, mice were monitored for weight loss (b). Two days post-challenge, at peak viral load, lung viral titers (c) and hemorrhage (0 = no hemorrhage, 4 = severe hemorrhage in all lobes) (d) were assessed from 5 animals per group. (a) Statistical analysis was not performed. (c-d) All dose levels were compared by Kruskal-Wallis ANOVA with Dunn’s multiple comparisons test. (b) For weight loss, all comparisons are to PBS control mice at each timepoint by two-sided Mann-Whitney test. ** = p-value < 0.01, **** = p-value < 0.0001. Data are presented as GMT +/− geometric SD (a,c) or mean +/− SD (b,d). (c) Dotted line represents assay limit of detection.