Extended Data Figure 10. Flow cytometry panel to quantify SARS-CoV-2 S-specific T cells in mice.

(a) Related to Figure 3d-g, hierarchical gating strategy was used to unambiguously identify single, viable CD4+ and CD8+ T cells. Gating summary of SARS-CoV-2 S-specific (b-c) CD4+ and (d-e) CD8+ T cells elicited by 0.1 and 1 μg mRNA-1273 immunization. Antigen-specific T cell responses following peptide pool re-stimulation were defined as CD44^hi/cytokine⁺. Concatenated files shown were generated using the same number of randomly selected events from each animal across the different stimulation conditions using FlowJo software, v10.6.2.