Fig. 6.

Increased arginase activity and apparent NOS uncoupling in MAF cells. a Intracellular arginase activity of MAF and DF cells derived from the same patients were compared in cell lysates. Results shown were normalized to total protein content (min to max; p = 0.0186, n = 5, ratio paired t test) (data from two independent experiments). b MAF and DF cells from the same patients were stained with DAF-FM and the frequency of NO producing cells was assessed (min–max; p = 0.0113, n = 5, paired t test, data from two independent experiments). Significant difference was found in the amount of produced NO (MFI) per cell, too (p = 0.0021, data not shown). c, d Evaluation of IL-IDO activity/l-kynurenine production, IL-6 and CXCL12 release (paired t test, p = 0.0178, n = 8, one experiment) in MAF and DF cells by ELISA