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. Author manuscript; available in PMC: 2021 Jan 27.
Published in final edited form as: Nat Microbiol. 2020 Jul 27;5(11):1319–1329. doi: 10.1038/s41564-020-0763-4

Extended Data Fig. 1. UV-LC-MS detection of colipterins 1–8 produced by the probiotic E. coli Nissle1917.

Extended Data Fig. 1

a, Dose-responses of 1-8 from the probiotic E. coli Nissle1917 exposed to the sub-lethal levels of sulfamethoxazole (SMX). Intensity was determined by extracted ion counts (EICs) with m/z corresponding to each colipterins within a 10 ppm error window. High-resolution ESI-QTOF-LC-MS spectra of samples were analyzed using Phenomenex Kinetex C18 (100Å) 5 μm (250 × 4.6 mm) column with a gradient from 10%−100% aqueous acetonitrile in 0.1% formic acid over 30 min and with a 0.7 ml min−1 flow rate. Data were collected in biological triplicate, and representative EIC chromatograms are shown. b, UV-Vis spectra of colipterins 1-8 and pterin.