Fig. 2.

(A) Profile mass spectra of the [M + 2H]²⁺ ion of an N-glycan with composition Hex₅HexNAc₄Fuc₁NeuAc₁ from undifferentiated NTERA-2 cells. Because doubly charged species are shown, the mass shift due to ¹³C labeling is 0.5 Da per labeled carbon. The mass spectra are corrected for the natural isotope distribution of carbon; the corresponding corrected isotopologue data are shown in the first column of (B). Corrected isotopologue data are also shown for the following representative glycans of each type of glycoconjugate: (B) N-glycan, Hex₅HexNAc₄Fuc₁NeuAc₁; (C) O-glycan, Hex₂HexNAc₂NeuAc₁; and (D) GSL, GM2(d34:1). Plots are shown for cells grown with regular glucose (ctrl) and [¹³C-UL] monosaccharides (5 mM glucose, 5 mM galactose, 5 mM mannose, 5 mM fructose and 100 uM fucose).