Figure 11.

Vaccination by either pOVA or pOVA-PEG20 confers partial tolerance in a Th1-driven DTH model. OVA-specific CD4⁺ cells were adoptively transferred into BALB/c mice, which were treated i.v. with PBS (control), 5 μg of pOVA or equimolar amounts of pOVA-PEG20 24 h later. On day 7, in vitro generated OVA-specific Th1 cells were transferred into the recipients. After 24 h, pOVA in IFA or the control, PBS/IFA, were injected into the left or right hind footpad, respectively. The difference in footpad thickness after challenge was monitored for eight consecutive days. Statistical testing was performed using the nonparametric Mann Whitney test and Holm-Bonferroni correction for multiple comparisons. Difference between pOVA-PEG20 and pOVA was significant with adjusted p = 0.03. Data represent mean of Δ footpad thickness ± SEM (n = 15).