Figure 12.

Non-hematopoietic APCs present pOVA-PEG20 and induce activation of OVA-specific T cells at high doses. OVA-specific CD4⁺ MHCII^-/^- cells, isolated from OTIIxB6.PL (CD90.1⁺) mice, were labeled with CFSE and transferred into controls or MHCII deficient BM (bone marrow) chimeras. After 24 h, recipients received 5 or 50 µg (based on peptide amount) of pOVA-PEG20. Proliferation as well as Foxp3 expression was assessed using flow cytometry on day 7. Splenocytes were gated on donor (CD90.1⁺) OVA-specific CD4⁺ T cells. Representative FACS dot plots from at least two independent experiments. wt: wild type.