Figure 8.

Administration of pOVA-PEG20 reduces the frequency of OVA-TCR⁺ CD4⁺ T cells among total CD4⁺ splenocytes. OVA-specific CD4⁺ cells were transferred into BALB/c mice. After 24 h, mice received i.v. PBS (control), 5 µg of pOVA or equimolar amounts of pOVA-PEG conjugates. After 6 days, the frequency of OVA-TCR⁺ CD4⁺ cells among total CD4⁺ T cells was assessed using flow cytometry. Mean ± SD of % KJ1.26⁺ cells (OVA-TCR⁺ T cells) among total CD4⁺ splenocytes from at least two independent experiments. n = 5–11; PBS, n = 13. Statistical testing was performed using the nonparametric Mann Whitney test and Holm-Bonferroni correction for multiple comparisons. ns, non-significant, *p < 0.05; **p < 0.01; ***p < 0.001.