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. 2020 Oct 22;11(10):894. doi: 10.1038/s41419-020-03029-0

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© The Author(s) 2020

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Fig. 6 — a The motif analysis was conducted to ChIP-seq of MNX1. b ChIP assay was performed to determine whether MNX1 bound to the promoter of Ajuba. The result indicated that MNX1 bound to the promoter of Ajuba (P2 and P3). c The expression of essential molecules in the Hippo signaling pathway after upregulating or downregulating the expression of MNX1-AS1 or MNX1 by western blot assay. The results indicated that overexpression of MNX1 facilitated the expression of Ajuba protein, and subsequently repressed the activity of the Hippo pathway, the western blot assay was repeated three times for each group. (***P < 0.001 by t-test).