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. 2020 Oct 22;11(10):892. doi: 10.1038/s41419-020-03106-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, b Cell viability of MCF7 and T47D cells under the indicated treatments. Values are means ± SD, ***p < 0.001 (Student’s t-test). c, d Colony formation assay of MCF7 and T47D cells under the indicated treatments. Values are means ± SD, ***p < 0.001 (Student’s t-test). Scale bar is 5 mm. e, f The OCR of MCF7 and T47D cells under the indicated treatments (n = 3). O: Oligomycin, F: FCCP; A&R: antimycin A/rotenone. g Spare respiratory capacity of MCF7 and T47D cells under the indicated treatments. Values are means ± SD, *p < 0.05; **p < 0.01; ***p < 0.001 (Student’s t-test). h Morphologic characteristic of subcutaneous xenograft tumors from MCF7/shNC + vector-TET-on group, MCF7/shTIM+vector-TET-on group, MCF7/shTIM + oeACER2-TET-on group (n = 5). Scale bar is 10 mm. i Tumor volume at day 25 was measured in each group. Values are means ± SD, **p < 0.01 (Student’s t-test). j Tumor weight at day 25 was measured in each group. Values are means ± SD, *p < 0.05. k Representative images of Ki67 staining in xenograft from each group. Scale bar is 50 mm.