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. 2020 Oct 22;10:18094. doi: 10.1038/s41598-020-75275-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Voltage of tissue samples during stimulation with the following solutions. (a) Ringer solution as an incubation and bathing fluid, as well as a mechanical stimulation—the control group. (b) Bumetanide solution as an incubation and bathing fluid, as well as a mechanical–chemical stimulation—the group of inhibited chloride ion transport. (c) Amiloride solution as an incubation and bathing fluid, as well as a mechanical–chemical stimulation—the group of inhibited sodium ion transport. Time-courses of single experiments are presented, showing the most frequently observed electrophysiological responses to stimulation. The arrows indicate mechanical (Ringer solution) or mechanical–chemical stimulation (bumetanide or amiloride).