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. 2020 Oct 22;10:18033. doi: 10.1038/s41598-020-75125-2

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© The Author(s) 2020

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Brain spheroids after 24 h hypoxia. Top: Live-dead assay with calcein AM (green; living cells) and ethidium (red; dead cells). Bottom: Fluorescence signal from Image-iT Green Hypoxia Reagent. Scale bars 200 µm.