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. 2020 Oct 9;11:575390. doi: 10.3389/fphar.2020.575390

Figure 1.

Figure 1

Metformin (MET) recovers gestational diabetes mellitus (GDM)-impaired the migration and angiogenesis of HUVECs. (A) A scratch wound assay was performed in the presence of 5.5 or 35 mM D-Glucose with or without MET (100 μM) for 72 h. Cell monolayers were imaged at 0, 24, 48, and 72 h after wounding. White vertical lines indicate the wound area borders. Space bar=500 μm. The images shown here were captured at 0 and 24 h. The cell migration distance was measured and shown on the right. (B) Tube formation assay was performed to assess the angiogenesis ability of HUVECs in the presence of 5.5 or 35 mM D-Glucose with or without MET for 72 h. Bars=50 mm. The tube length was quantified and shown on the right. All values displayed are mean ± SEM of 8 independent experiments. **P < 0.01, ***P < 0.001 vs. 5.5 mM D-Glucose group; ###P < 0.001 vs. 35 mM D-Glucose group.