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. 2020 Oct 9;11:575390. doi: 10.3389/fphar.2020.575390

Figure 2.

Figure 2

The role of Nrf2 in tube formation under the gestational diabetes mellitus (GDM) (HG) condition. (A) Western blot analysis of the Nrf2 level in normal and GDM placenta tissues and HUVECs. The Nrf2 protein levels were quantified and shown on the right (n=5). (B) Quantification of Nrf2 expression level in peripheral blood from normal and GDM pregnant women using Enzyme-Linked Immunosorbent Assay (ELISA). (C) Nrf2 expression level in normal and GDM HUVECs. (n=5). (D) Tube formation assay was performed to assess the angiogenesis ability of HUVECs in the presence of 5.5 or 35 mM D-Glucose with or without ML385 or THBQ for 72 h. HUVECs were cultured either in normal glucose (5.5 mM) or high glucose (35 mM) medium in the presence or absence of MET (100 μM) for 72 h. Bars=50 mm. All values displayed are means ± SEM of 8 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 vs. 5.5 mM.