FIGURE 3.

Determining the minimal attP and attB sites utilized by Int_Φ24B. (A) Diagram depicting the in situ assay setup, the arabinose induction of Int_Φ24B expression, and the resulting chimeric plasmid. (B) The design of the various attP constructs (plasmids with nomenclature pΦ24_B-attP, Supplementary Table 1), providing information on the lengths of the P (solid purple) and P’ (hashed purple) sequences and drawn to scale as they flank the 24 bp central crossover site (green). The detection of recombination (+) or absence (–) is also indicated. (C) The design of the various attB constructs (plasmids with nomenclature pΦ24_B-attB, Supplementary Table 1), providing information on the lengths of the B (solid blue) and B’ (hashed blue) sequences and drawn to scale as they flank the 24-bp central crossover site (red). The detection of recombination (+) or absence (–) is also indicated.