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. 2020 Oct 9;8:587685. doi: 10.3389/fcell.2020.587685

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Copyright © 2020 Gupta, Ambaru and Bajaj.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunofluorescence micrographs (A–F) after staining the cells with anti-LdTwf antibodies, showing movement of twinfilin (Twf) from the nucleolus to origin of the mitotic spindle where it completely localized on the extending spindle microtubules and finally redistributed to the spindle poles. Arrow heads mark distribution patterns of TWF on the spindle, showing the presence of residual TWF on the spindle microtubules while the larger TWF bulk migrated to the poles in the later stages of karyokinesis. Mitotic spindle has been marked by anti α-tubulin (aTub) antibody. Bar, 5 mm (taken from Kumar et al., 2016 with permission).