TABLE 2.
Mutations potentially contributing HCV permissiveness to Huh7 and Huh7.5.1-8.
| Gene | Status | Type* | Ensembl transcript (AA type) | Function† |
| BAX | Huh7.5.1-8-predominant | CID | N/A | 2 |
| COL6A3 | Huh7.5.1-8-predominant | SSM | N/A | 7 |
| DEFB104B | Huh7.5.1-8-predominant | CID | N/A | 6 |
| SIRPB1 | Huh7.5.1-8-predominant | GFM | N/A | 6 |
| ACIN1 | Shared | CID | N/A | 2 |
| AR | Shared | CID | N/A | 5 |
| ATG3 | Shared | CID | N/A | 1 |
| ATM | Shared | SDA | N/A | 1, 2 |
| ATP6AP2 | Shared | CID | N/A | 2 |
| CD34 | Shared | CID | N/A | 5 |
| CHMP4A | Shared | CID | N/A | 1, 2 |
| CTSA | Shared | CID | N/A | 1 |
| EGF | Shared | CID | N/A | 5, 4 |
| EIF2AK3 | Shared | CID | N/A | 5 |
| EIF4G3 | Shared | CID | N/A | 1 |
| GPX1 | Shared | CID | N/A | 2 |
| H3CR1 | Shared | CID | N/A | 5 |
| HTT | Shared | CID | N/A | 1, 2 |
| IFNA10 | Shared | SSM | N/A | 3, 5, 6 |
| IFT20 | Shared | SDA | N/A | 1 |
| KIR2DL4 | Shared | CID, SDA | N/A | 5 |
| M6PR | Shared | CID | N/A | 4 |
| MAP3K1 | Shared | CID | N/A | 2 |
| MMP17 | Shared | CID | N/A | 5 |
| NOTCH4 | Shared | CID | N/A | 5 |
| OVOS2 | Shared | CID | N/A | 7 |
| PHLDA1 | Shared | CID | N/A | 2 |
| PHLPP1 | Shared | CID | N/A | 2 |
| PRUNE2 | Shared | CID | N/A | 2 |
| PTEN | Shared | SDA | N/A | 2 |
| SNCA | Shared | SDA | N/A | 1 |
| TMBIM4 | Shared | GFM | N/A | 2 |
| TNRC18 | Shared | CID, SSM | N/A | 7 |
| TP53 | Shared | CID | N/A | 5, 2, 4 |
| UBE2D3 | Shared | CID | N/A | 2 |
| DDX58 | Huh7.5.1-8-predominant | Missense | ENST00000379883.2 (T55I) | 3, 5, 6 |
| EP300 | Huh7.5.1-8-predominant | Missense | ENST00000263253.8 (D985G) | 1 |
| EP300 | Huh7.5.1-8-predominant | Missense | ENST00000263253.8 (V1764I) | 1 |
| SPP1 | Huh7.5.1-8-predominant | Missense | ENST00000508233.5 (K241T) | 5 |
| ZNF654 | Huh7.5.1-8-predominant | Missense | ENST00000636215.1 (D1072N) | 4 |
| ASAH2 | Shared | Missense | ENST00000489640.5 (C74Y) | 2 |
| ATP6V0A2 | Shared | Missense | ENST00000613625.4 (G82E) | 1, 7 |
| C4A | Shared | Missense | ENST00000498271.1 (S347Y) | 6 |
| CASP8AP2 | Shared | Missense | ENST00000551025.3 (K1245N) | 2 |
| CYLD | Shared | Missense | ENST00000427738.7 (T822N) | 6 |
| DAB2IP | Shared | Missense | ENST00000408936.7 (R1164C) | 6, 2 |
| DIDO1 | Shared | Missense | ENST00000266070.8 (S1019C) | 2 |
| DPF2 | Shared | Missense | ENST00000531989.1 (G41V) | 2 |
| IL4R | Shared | Missense | ENST00000563002.5 (A82T) | 5 |
| PAK4 | Shared | Missense | ENST00000360442.7 (R175W) | 2 |
| PIP4K2C | Shared | Missense | ENST00000540759.6 (A300G) | 1 |
| TBC1D25 | Shared | Missense | ENST00000376771.8 (N277S) | 1 |
| TNFRSF10B | Shared | Missense | ENST00000276431.8 (V191A) | 5, 2 |
| TP53 | Shared | Missense | ENST00000509690.5 (Y88C) | 5, 2, 4 |
| TRIM56 | Shared | Missense | ENST00000306085.10 (R395W) | 3, 6 |
*Classes of LoF mutations: SDA, splice donor/acceptor mutation; GFM, gene fusion mutation; CID, coding sequence InDel; Missense, missense mutation; SSM, start/stop codon mutation. †1, autophagy-related gene; 2, apoptosis-related gene; 3, antivirus-related gene; 4, genes involved in protein-protein association networks, with nine core genes that showed strong influence on HCV infection and replication; 5, known hepatitis C-related gene; 6, innate immune-related gene; 7, serine protease-related gene.