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WT1 silencing (WT1-siRNA) induced apoptosis via activation of caspase-3/7 enzyme activity of K562 cell (K562-WT1-siRNA-GFP+ cells) at the specific time points compared with the C-siRNA-GFP+ control cells (C-siRNA). Analysis of caspase-3/7 enzyme activities was performed by using the Apo-One Homogeneous Caspase-3/7 apoptosis kit (Promega).
