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WT1 silencing (WT1-siRNA) downregulated the expression of IL-2, IL-2RB, and IL-2RG compared with the control cells (C-siRNA). RT-PCR analysis of IL-2, IL-2RB, and IL-2RG mRNA was performed on RNA isolated from K562-WT1-siRNA-GFP+ or K562 cells after WT1-siRNA lentiviral vector transduction (WT1-siRNA) and K562-C-siRNA-GFP+ control cells (C-siRNA). GAPDH mRNA detection was used as an internal control.
