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. 2020 Oct 22;10:18061. doi: 10.1038/s41598-020-73005-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Platelet agonist stimulation facilitates the formation of EVs with no difference in average vesicle size according to NTA. (A) Head to head comparison of tracked vesicle concentration generated from either unstimulated (Buffer) platelets or platelets stimulated by the addition of TRAP-6. Vesicles generated from 1 ml of 3 × 10⁸ platelets in 1 ml of MTH buffer. Platelets were then removed and the supernatant injected on NTA. Two-tailed paired t-test, p = 0.001, n = 11. (B) Comparison of tracked vesicle average size. No significant difference was observed from both types of vesicles: Buffer PDEVs = 191.6 nm × TRAP-6 PDEVs = 190.3 nm. Results are presented as Mean ± SD, n = 8. Two-tailed paired t-test p = 0.663, n = 11.