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. 2020 Oct 22;10:18061. doi: 10.1038/s41598-020-73005-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Circulating and PDEVs do not express TF. To assess whether EVs from different sources could stimulate thrombin generation in the absence of TF, 10 µg/ml of all types of EVs studied here were added to normal plasma, in the presence and absence of synthetic phospholipid membranes (4 µM). The peak thrombin generation was compared to that generated by normal plasma alone in the presence of 4 µM phospholipids and 1 pM TF. Results presented as Mean ± SD, n ≥ 3.