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. 2020 Oct 22;10:18102. doi: 10.1038/s41598-020-74853-9

Figure 1.

Figure 1

Dosing and testing paradigm used for perinatal and adult exposure to DE-71. Direct exposure to DE-71, represented by solid shading in adult dams (F0♀), began ~ 4 weeks pre-conception and continued daily for ~ 3 weeks of gestation and 3 weeks of lactation until pup weaning at PND 21. Indirect exposure to DE-71, represented by hatched shading, in female offspring (F1♀) occurred perinatally (ED 0 to GD 18) and postnatally via lactation through weaning (PND 0-PND 21) for ~ 39 days. Maternal and litter parameters were taken during GD 15 to PND 0. Using this regimen, exposure duration was 70–80 days for F0 and 39 d for F1. Metabolic endpoints (fasting glycemia, IPGTT, ITT) were examined in adulthood, at 1–2 weeks post-weaning for dams (at ~ 5 months old) and ~ 4 months for offspring. At necropsy, body and organ weights were recorded and blood and organ tissues collected for further analysis: GC/ECNI/MS BDE congener determination, plasma ELISA, UPLC/MS/MS detection of endocannabinoids, epinephrine assay, liver enzymatic activity. GD gestational day, PND postnatal day, ED embryonic day. This figure was created by E.V.K. using Microsoft PowerPoint for Mac Version 16.27 (https://www.microsoft.com/en-us/microsoft-365/p/powerpoint).