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. 2020 Oct 22;10:18083. doi: 10.1038/s41598-020-75048-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Functional characterization of gene expression in the venom gland relative to other non-venom secretory tissues. (a) Venn diagram denoting shared and unique genes between all pairwise comparisons. (b) Inferences of activated canonical pathways based on differentially expressed genes between non-venom tissues and the unextracted venom gland (green), with inferred activity in analyses of unextracted versus 1DPE venom gland tissues shown in grey if present. (c) Top 25 inferences of activated URMs based on differentially expressed genes between non-venom tissues and the unextracted venom gland (green), with inferred activity in analyses of unextracted versus 1DPE venom gland tissues shown in grey if present. (d) Significantly enriched GO terms of genes significantly upregulated in the unextracted venom gland relative to body tissues. All terms shown are significantly enriched (p < 0.05), and node size is inversely proportional to enrichment p-value, with larger nodes having a lower p-value. Connected nodes indicate a high proportion of shared genes underlying term enrichment.