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. 2020 Oct 22;10:17995. doi: 10.1038/s41598-020-75057-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Screening of RNA-binding proteins (RBPs) that regulate the alternative splicing of TREM2 exon 3. (A) Schematic diagram of the fl-TREM2 minigene. The arrows indicate the primer set to detect the splicing pattern of exon 3. (B) Results of the splicing assay using the fl-TREM2 minigene and a panel of RBPs. RBPs were expressed as a fusion with EGFP. Splice products were detected by RT-PCR using agarose gels. The proportion of exon 3 skipping is indicated at the bottom of each lane. EGFP (EV) and pcDNA3.1 were used as controls. (C) The effect of human CELF proteins (CELF1/2/3/4) on the splicing of TREM2 exon 3. The fl-TREM2 minigene was co-transfected with EGFP-fused CELF proteins into HEK cells. Bar chart shows the proportion of exon 3 skipping. Error bars represent SD (n = 3). Tukey’s test was used for statistical evaluation.