Figure 2.

(A) Sequencing chromatograms corresponding to the predicted promoter region of the hns2 gene of strain 042 WT and one of the lineages of the hns mutant derivative at different subculture intervals. The -10 sequence of the hns2 promoter is indicated with a gray box, whereas the -35 sequence is indicated in blue. A black box highlights the C > T transition generated in the hns2 promoter. (B) Sequencing chromatograms corresponding to the -10 sequence of the hns2 gene promoter region of the 042 WT strain and of the three different lineages of the hns mutant derivative upon 5 and 10 days subculture at 25 °C. The blue circle highlights the C present in the WT promoter, whereas the red circle highlights the transition to T.