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. 2020 Oct 22;10:18017. doi: 10.1038/s41598-020-73708-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Representative fluorescence images of the biofilms treated with DMSO (control), glycolipid 3 (treatment) and drug control (cipladine). Biofilm formed by MRSA, MSSA, and PA was stained with SYTO9 and PI. Green colour represents live cells and red represents the dead cells. Scale bar is 250 µm; (b) Quantification data obtained from fluorescent microscopic images of biofilms formed from MRSA, MSSA and PA. N = > 20. Mann–Whitney U test was performed to analyze the data (***P < 0.001).