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. 2020 Oct 22;11:5348. doi: 10.1038/s41467-020-18998-1

Fig. 3. PCLX-001 treatment results in SFK instability and degradation by the proteasome in lymphoma cell lines.

Fig. 3

Western blot for total Src and Lyn proteins in immortalized lymphocyte (IM9, VDS), BL (BL2, Ramos, BJAB), and DLBCL (DOHH2, WSU-DLCL2, SU-DHL-10) cell line lysates following 24–48 h of treatment with 0.1 µM or 1.0 μM PCLX-001 (a). After BCR ligation with anti-IgM, western blot for total Src, Lyn, Hck, Lck, Mcl-1, total phospho-tyrosine (PY99) and pan phosphorylated-SFK (P-SFK) protein levels in BL2 treated for 24–48 h with 1 µM PCLX-001 in the presence or absence of 10 µM of the proteasome inhibitor MG132 for the last 6 h (b). GAPDH serves as a loading control. All western blots shown are representative of three independent experiments. Source data are provided as a Source Data file.