Figure 4.

Acidic pH inhibited NLR family CARD domain-containing 4 (NLRC4) inflammasomes and absent in melanoma 2 (AIM2). (a–d) IL-1β levels by ELISA assay after wild type (WT) iBMDMs (a,b) or NLRP3^−/− iBMDMs (c,d) were primed with 100 ng/mL LPS for 6 h and then exposed to buffers with various pH values alone, containing 3 μg/mL Saty flagellin with DOTAP transfection reagent (a,c) or 2 μg/mL poly(dA:dT) with Lipofectamine transfection reagent (b,d) for 3 h.