Figure 2.

Inhibition of Bluetongue virus (BTV) and African horse sickness virus (AHSV) by ATA. (a) Cytotoxicity of ATA. Vero cells were treated with the indicated doses (two-fold dilutions, starting concentration 1000 μM) of ATA. Cell viability was analyzed at 24 h after treatment. Data are expressed as mean and SD. (b) Schematic representation of the experimental design. Vero cells were infected (multiplicity of infection (MOI), 0.01) with BTV-4 (c) or AHSV-4 (d), and after 90 min of viral adsorption, the indicated concentrations (two-folds dilutions, starting concentration 1000 μM) of ATA were added to the infection media (0 h.p.i.). At 24 (left) and 48 (right) h post-infection (h.p.i)., tissue culture supernatants were collected for virus titration by the plaque assay. The IC₅₀ is indicated. Data are expressed as mean and SD. Dotted lines indicate the limit of detection.