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The protein expression of hepatic IGF-1 and IGFBP-3. Liver extract from Sprague-Dawley rats 14 days after treatment were analyzed via western blotting with anti-IGF-1 (A) and anti-IGFBP-3 (C) antibodies. β-actin served as the loading control. (B,D) Quantification of IGF-1 and IGFBP-3 levels. Data are expressed as means ± SD (n = 6). * p < 0.05, ** p < 0.01 and, *** p < 0.001 vs. normal. IGF-1, insulin-like growth factor-1; IGFBP-3, insulin-like growth factor binding protein-3.
