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. 2020 Sep 23;25(19):4375. doi: 10.3390/molecules25194375

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Immunohistochemical localization of bone morphogenic proteins in the proximal tibial growth plate. BMP-2 (A) and BMP-4 (C) were detected in the proliferating and hypertrophic zone of the proximal tibial growth plate. Scale bar; 50 μm. (B,D) The stained area of BMP-2 and BMP-4 was analyzed using ImageJ^® and calculated in terms of the fold of the control. Data are expressed as means ± SD (n = 6). ** p < 0.01 and *** p < 0.001 vs. normal. BMP-2, bone morphogenetic protein-2; BMP-4, bone morphogenetic protein-4; PZ, proliferating zone; HZ, hypertrophic zone.