Figure 3.

Cell membrane reorganization was radio-induced in radiosensitive SCC61 cells but not in radioresistant SQ20B cells. ASMase activation and ceramide production were measured using immunofluorescence in (A) SCC61 cells (donor and recipients) and (B) SQ20B cells (donor and recipients). (C) ASMase and ceramide levels in basal condition (untreated) in SCC61 and SQ20B cells were quantified using immunofluorescence images. (D) Lipid raft markers were assessed using western blotting in both SCC61 and SQ20B cells in the total lysate or in the fractionated Triton-soluble membrane (non-rafts) and lipid raft (rafts) fractions as described. (E) Cholesterol level was measured in non-raft and raft fractions isolated from SCC61 and SQ20B cells in basal conditions. The results are the mean ± SD of three experiments performed in triplicate. * p < 0.05, ** p < 0.01.