Skip to main content
. 2020 Sep 29;21(19):7200. doi: 10.3390/ijms21197200

Figure 4.

Figure 4

Role of lipid raft formation in radiosensitive SCC61 cells: SCC61 donor cells were preincubated for 30 min with 4 mM methyl-β-cyclodextrin (MBCD; lipid raft disruptor) before irradiation in the presence of MBCD. After irradiation, the medium was removed and (A) clonogenic survival was determined in donor cells and in recipient cells. (B) The number of micronuclei per cell was determined in donor cells in the presence or absence of MBCD and in their corresponding recipient cells. (C) Apoptosis was measured by a TUNEL assay in SCC61 donor cells preincubated or not with 4 mM MBCD for 30 min before irradiation. The results are the mean ± SD of three experiments performed in triplicate. * p < 0.05, ** p < 0.01, and *** p < 0.001 compared with cells treated only with radiation. (D) The expression of cytokines secreted in conditioned medium from untreated, irradiated (1.5 Gy), and MBCD + irradiated SCC61 cells was evaluated using the Human Cytokine Array (Proteome Profiler Array; R&D Systems, Minneapolis, MN, USA). For each cytokine, ImageJ software was used to determine pixel intensity.