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. 2020 Sep 29;21(19):7200. doi: 10.3390/ijms21197200

Figure 6.

Figure 6

An endogenous AKT protein resides in lipid raft fractions of SQ20B cells and confers resistance to targeted and nontargeted effects. (A) SQ20B cells treated without (untreated) or with MBCD were incubated with Alexa-488-conjugated cholera toxin B (CTxB) following incubation with p-AKT S473. (B) SQ20B cells were pretreated in the presence or absence of MBCD, and total cell lysates (upper panel) or fractions isolated from non-raft and raft domains (bottom panel) were subjected to SDS-PAGE followed by western blotting for p-AKT S473. (C) SQ20B cells were preincubated or not with the phosphatidylinositol-3-kinase (PI3K) inhibitor (0.5 µM wortmannin) for 60 min followed by irradiation (donor cells) or CM from SQ20B donor cells (recipient cells), and clonogenic survival was determined. The results are the mean ± SD of three experiments performed in triplicate. * p < 0.05, ** p < 0.01, compared with cells treated only with radiation. (D) p-AKT S473 was expressed in total cell lysates of SQ20B and SCC61 cells in the presence or absence of MBCD or wortmannin.