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. 2020 Sep 30;21(19):7230. doi: 10.3390/ijms21197230

Figure 2.

Figure 2

Representative microphotographs of immunohistochemical staining for (a,b) SGLT1 (ab14685, Abcam) [55], (c,d) SGLT2 (NBP1-92384, Novus Biologicals, Centennial, CO, USA) [55], and (e) SGLT2 (ab85626, Abcam, 5 μg/mL, antigen retrieval with citrate buffer (pH 6)) in autopsied human brains. Endothelial cells of brain microcapillaries express neither SGLT1 (a) nor SGLT2 (c). CP epithelial cells are devoid of SGLT1 immunoreactivity (b). In contrast, SGLT2 is expressed in CP epithelial cells, with an intracytoplasmic granular pattern (d), or along the plasma membrane of the basal side (e, arrows), depending on the antibodies used. Scale bars: 50 μm.