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Characterization of human-iPSC generated NSCs by immunocytochemistry (ICC) and gene expression analysis. (A) Schematic diagram of iPSC to NSC differentiation. (B) ICC analysis of generated NSCs showing expression of NSC markers Nestin, PAX6, SOX1 and SOX2. (C) Correlation coefficient (r2) plot based on all expressed mRNAs between six iPSCs and their differentiated NSCs. (D) Gene expression plot showing dorsal neuroepithelial transcriptomic profile of generated NSCs.
