Table 3.
The mobile phase (0.1% tetrafluoroacetic acid in H2O: methanol) gradient.
| Time (min) | Tetrafluoroacetic Acid in H2O (%) | Methanol (%) |
|---|---|---|
| 0.0 | 70.0 | 30.0 |
| 1.0 | 70.0 | 30.0 |
| 5.00 | 0.0 | 100.0 |
| 6.00 | 0.0 | 100.0 |
| 10.00 | 70.0 | 30.0 |
| 2.00 | 70.0 | 30.0 |
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