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Single-cell sorting using DEPArray. (A) Flow cytometry analysis of CD34-positive CD38-negative (CD34+CD38+) cells fixed with 2% PFA overnight. As shown, APC and PE fluorochromes are compatible with PFA. (B) Single CD34+/CD38- cells sorted by DEPArray, labeled with anti-CD34-APC and anti-beads-PE. The labeling check reagent-PE identifies CD38 expression. All cells have been subjected to DAPI staining in order to assess their viability.
