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. 2020 Oct 6;21(19):7383. doi: 10.3390/ijms21197383

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Sequence profiles of HSVd-sRNA derived from the genomic (+) and anti-genomic (−)-strands of HSVd-g or HSVd-h in cucumbers. The top panel (A) shows the predicted secondary structure of HSVd-g. The five hop-adaptive mutations between HSVd-g and HSVd-h are indicated by the arrows. sRNA distribution profiles shown in panel (B) represent the sum of 21–24-nt HSVd-sRNA populations recovered from cucumber plants infected with HSVd-h or HSVd-g plotted along the linearized HSVd genome (nucleotides 1–297 from left to right). sRNA distribution profiles of HSVd-g and HSVd-h are compared for genomic (upper panel) and anti-genomic (lower panel) strands. Genome positions of selected hot-spot peaks are marked by corresponding numbers. Arrowheads indicate sRNAs containing adaptive mutations that distinguish HSVd-g from HSVd-h and accumulate to obviously different levels. Details of their amounts and sequences are shown in panel (C).