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. 2020 Oct 3;21(19):7328. doi: 10.3390/ijms21197328

Figure 1.

Figure 1

(a) Hierarchical Clustering Heatmap analysis of miRNAs detected (n = 119) in at least 4/5 sample for each group. A total of 15 subjects were analyzed: n = 5 non diabetic controls (CTR), n = 5 type 2 diabetic patients (D) and n = 5 type 2 diabetic with Diabetic Macular Edema patients (DME). MicroRNAs expression levels are reported as scale colors based on ΔCT expression (Blue, high expression: ΔCT = −3; Red, low expression: ΔCT = 2. (b) Venn diagram showing miRNAs distribution among the three groups; in particular, 83 miRNAs were common among the three groups, while 19 miRNAs were exclusively found in CTR and D but not in DME group; moreover, four miRNAs were exclusively detected in CTR groups and 10 miRNAs resulted exclusively expressed in D group, while only 1 miRNA is exclusively detected in DME group. (c) Stability ranking of top 10 candidate reference miRNAs based on SSS metric which combined three different assessment algorithms. Most stable miRNAs presented lowest SSS values.