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. 2020 Oct 8;25(19):4590. doi: 10.3390/molecules25194590

Figure 8.

Figure 8

Aromadendrin induces the Nrf2 pathway and acts as an antioxidant in activated T cells. (A) The expression of HO-1 was detected by Western blot in Jurkat T cells treated with 20 or 40 μM of aromadendrin for 24 h. (C) The expression of Nrf2 in the cytosol and nucleus was determined by Western blot in Jurkat T cells treated with 20 or 40 μM of aromadendrin for 2 h. To separate nuclear extracts from whole lysate, a PE-NER kit was used. (E) Generated ROS (reactive oxygen species) was measured by 10 μM DCF-DA staining in Jurkat T cells pre-treated with 20 or 40 μM of aromadendrin for 24 h and treated with PMA (100 nM)/A23187 (1 μM) for 1 h. (G) Jurkat cells pre-treated with 40 μM aromadendrin for 24 h was stimulated with PMA (100 nM)/A23187 (1 μM) for 12 h or 24 h. After stimulation, cells were harvested, and the expression of catalase (CAT) and superoxide dismutase (SOD) was detected by Western blot. (B,D,F,H) The mean values ± SEM are presented in the bar graph. * p < 0.05, versus the control cells (A,C,E) or indicated two groups (G).