Figure 5.

Snapshots showing single nucleotide polymorphism (SNP) genotyping in parental inbred line LM11, CML25, and their F₂ mapping populations with KASP marker assays. The snapshots show (a) polymorphic pattern, occurrence of allele specific to LM11 (blue spots) and CML25 (red spots), and heterozygosity for the corresponding alleles (green spots) in F₂ mapping population. (b) occurrence of only one allele (blue spots), and heterozygosity for corresponding allele (green spots). (c) monomorphic pattern, occurrence of allele specific to LM11 (blue spots). (d) monomorphic pattern, occurrence of allele specific to CML25 (red spots), and missing data (pink spot). (e) the majority of missing data (pink spots), and one allele (red spots), occurrence of failed amplification signals i.e., invalid markers, in all snapshots black spots represent no template controls (NTCs).