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. 2020 Sep 27;21(19):7126. doi: 10.3390/ijms21197126

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Validation the targeting regulation of GhBLH7-D06 on GhPAL-A06: (a,b) the targeting regulation of GhBLH7-D06 and GhOFP3-D13 on GhPAL-A06 by dual-luciferase reporter assay system; and (c) validation of interaction between GhBLH7-D06 and GhPAL-A06 promoter by Y1H. The empty vector control group (pHIS2-pGhPAL-A06 + pGADT7) and the experimental group (pHIS2-pGhPAL-A06 + pGADT7-GhBLH7-D06) were grown on two-deficient medium SD-T/L (SD-Trp/-Leu) and three-deficient medium SD-T/L/H + 150 mM 3-AT (SD-Trp/-Leu/-His + 150 mM 3-AT). Error bars represent the standard deviation of three biological replicates. Asterisks indicate statistically significant differences, as determined by Student’s t-test (** p < 0.01).