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. 2020 Sep 27;21(19):7120. doi: 10.3390/ijms21197120

Figure 1.

Figure 1

Detection of the candidate fusion gene MRPS31-SUGT1. (a) RT-PCR analysis of cDNA derived from pathologic tissues (P: polyp; T: tumor) and adjacent normal mucosa (N) in three CRC patients (#1, #2, #3). RT-PCR products were visualized on an agarose gel; (b) Sequencing analysis of the MRPS31-SUGT1 fusion transcript in patients. Sequencing electropherograms seem to reveal the fusion between exon 6 of MRPS31 and exon 3 of SUGT1 at the breakpoint; (c) the next check revealed that the amplicon corresponds to the MRPS31P5; the red arrows indicating the nucleotide difference between our fragment that correspond to the MRPS31P5 (100% similarity) and sequence of the exon 6 of MRPS31 (90% similarity gene.). The grouping of gels was cropped from different parts of the same gel.